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ebm-2 complete culture media  (Lonza)


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    Structured Review

    Lonza ebm-2 complete culture media
    Ebm 2 Complete Culture Media, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/complete+ebm-2+culture+media/ebm+2+medium/pmc10940855-40-7-9
    Average 90 stars, based on 1 article reviews
    ebm-2 complete culture media - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Isolation:

    Article Title: CircRNA ARFGEF1 functions as a ceRNA to promote oncogenic KSHV-encoded viral interferon regulatory factor induction of cell invasion and angiogenesis by upregulating glutaredoxin 3
    Article Snippet: Primary human umbilical vein endothelial cells (pri-HUVECs), which were used between passages 3 and 6, were isolated from the interior of the umbilical vein of human umbilical cords by digestion with collagenase (Sigma, St. Louis, MO, USA), and cultured in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) as previously described [ ]. pri-HUVECs were used for migration and invasion assays, a human umbilical vein endothelial cell line, EA.hy926 (catalog #CRL-2922; ATCC, Manassas, VA, USA) was employed for RNA-seq analysis, plate colony formation assay and in vivo matrigel plug assay, and HEK293T cells were used for lentivirus packaging and luciferase activity assay.

    Article Title: Viral interleukin-6 encoded by an oncogenic virus promotes angiogenesis and cellular transformation by enhancing STAT3-mediated epigenetic silencing of caveolin 1
    Article Snippet: Primary human umbilical vein endothelial cells (HUVECs) were cultured in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) as previously described [ 32 ].

    Article Title: HIV-1 Nef and KSHV oncogene K1 synergistically promote angiogenesis by inducing cellular miR-718 to regulate the PTEN/AKT/mTOR signaling pathway
    Article Snippet: Primary human umbilical vein endothelial cells (HUVECs) were cultured and maintained in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) as described ( ).

    Article Title: A KSHV microRNA Directly Targets G Protein-Coupled Receptor Kinase 2 to Promote the Migration and Invasion of Endothelial Cells by Inducing CXCR2 and Activating AKT Signaling
    Article Snippet: HUVEC were cultured in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) and used between passage 3 and 6.

    Article Title: Sperm associated antigen 9 promotes oncogenic KSHV-encoded interferon regulatory factor-induced cellular transformation and angiogenesis by activating the JNK/VEGFA pathway
    Article Snippet: HUVECs were cultured in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) and used between passage 3 and 6.

    Article Title: A KSHV microRNA enhances viral latency and induces angiogenesis by targeting GRK2 to activate the CXCR2/AKT pathway
    Article Snippet: HUVECs were cultured in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) and used between passage 3 and 6.

    Cell Culture:

    Article Title: CircRNA ARFGEF1 functions as a ceRNA to promote oncogenic KSHV-encoded viral interferon regulatory factor induction of cell invasion and angiogenesis by upregulating glutaredoxin 3
    Article Snippet: Primary human umbilical vein endothelial cells (pri-HUVECs), which were used between passages 3 and 6, were isolated from the interior of the umbilical vein of human umbilical cords by digestion with collagenase (Sigma, St. Louis, MO, USA), and cultured in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) as previously described [ ]. pri-HUVECs were used for migration and invasion assays, a human umbilical vein endothelial cell line, EA.hy926 (catalog #CRL-2922; ATCC, Manassas, VA, USA) was employed for RNA-seq analysis, plate colony formation assay and in vivo matrigel plug assay, and HEK293T cells were used for lentivirus packaging and luciferase activity assay.

    Article Title: Viral interleukin-6 encoded by an oncogenic virus promotes angiogenesis and cellular transformation by enhancing STAT3-mediated epigenetic silencing of caveolin 1
    Article Snippet: Primary human umbilical vein endothelial cells (HUVECs) were cultured in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) as previously described [ 32 ].

    Article Title: HIV-1 Nef and KSHV oncogene K1 synergistically promote angiogenesis by inducing cellular miR-718 to regulate the PTEN/AKT/mTOR signaling pathway
    Article Snippet: Primary human umbilical vein endothelial cells (HUVECs) were cultured and maintained in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) as described ( ).

    Article Title: A KSHV microRNA Directly Targets G Protein-Coupled Receptor Kinase 2 to Promote the Migration and Invasion of Endothelial Cells by Inducing CXCR2 and Activating AKT Signaling
    Article Snippet: HUVEC were cultured in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) and used between passage 3 and 6.

    Article Title: Sperm associated antigen 9 promotes oncogenic KSHV-encoded interferon regulatory factor-induced cellular transformation and angiogenesis by activating the JNK/VEGFA pathway
    Article Snippet: HUVECs were cultured in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) and used between passage 3 and 6.

    Article Title: A KSHV microRNA enhances viral latency and induces angiogenesis by targeting GRK2 to activate the CXCR2/AKT pathway
    Article Snippet: HUVECs were cultured in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) and used between passage 3 and 6.

    Migration:

    Article Title: CircRNA ARFGEF1 functions as a ceRNA to promote oncogenic KSHV-encoded viral interferon regulatory factor induction of cell invasion and angiogenesis by upregulating glutaredoxin 3
    Article Snippet: Primary human umbilical vein endothelial cells (pri-HUVECs), which were used between passages 3 and 6, were isolated from the interior of the umbilical vein of human umbilical cords by digestion with collagenase (Sigma, St. Louis, MO, USA), and cultured in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) as previously described [ ]. pri-HUVECs were used for migration and invasion assays, a human umbilical vein endothelial cell line, EA.hy926 (catalog #CRL-2922; ATCC, Manassas, VA, USA) was employed for RNA-seq analysis, plate colony formation assay and in vivo matrigel plug assay, and HEK293T cells were used for lentivirus packaging and luciferase activity assay.

    Article Title: Viral interleukin-6 encoded by an oncogenic virus promotes angiogenesis and cellular transformation by enhancing STAT3-mediated epigenetic silencing of caveolin 1
    Article Snippet: Primary human umbilical vein endothelial cells (HUVECs) were cultured in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) as previously described [ 32 ].

    Article Title: HIV-1 Nef and KSHV oncogene K1 synergistically promote angiogenesis by inducing cellular miR-718 to regulate the PTEN/AKT/mTOR signaling pathway
    Article Snippet: Primary human umbilical vein endothelial cells (HUVECs) were cultured and maintained in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) as described ( ).

    Article Title: A KSHV microRNA Directly Targets G Protein-Coupled Receptor Kinase 2 to Promote the Migration and Invasion of Endothelial Cells by Inducing CXCR2 and Activating AKT Signaling
    Article Snippet: HUVEC were cultured in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) and used between passage 3 and 6.

    Article Title: Sperm associated antigen 9 promotes oncogenic KSHV-encoded interferon regulatory factor-induced cellular transformation and angiogenesis by activating the JNK/VEGFA pathway
    Article Snippet: HUVECs were cultured in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) and used between passage 3 and 6.

    Article Title: A KSHV microRNA enhances viral latency and induces angiogenesis by targeting GRK2 to activate the CXCR2/AKT pathway
    Article Snippet: HUVECs were cultured in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) and used between passage 3 and 6.

    RNA Sequencing:

    Article Title: CircRNA ARFGEF1 functions as a ceRNA to promote oncogenic KSHV-encoded viral interferon regulatory factor induction of cell invasion and angiogenesis by upregulating glutaredoxin 3
    Article Snippet: Primary human umbilical vein endothelial cells (pri-HUVECs), which were used between passages 3 and 6, were isolated from the interior of the umbilical vein of human umbilical cords by digestion with collagenase (Sigma, St. Louis, MO, USA), and cultured in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) as previously described [ ]. pri-HUVECs were used for migration and invasion assays, a human umbilical vein endothelial cell line, EA.hy926 (catalog #CRL-2922; ATCC, Manassas, VA, USA) was employed for RNA-seq analysis, plate colony formation assay and in vivo matrigel plug assay, and HEK293T cells were used for lentivirus packaging and luciferase activity assay.

    Article Title: Viral interleukin-6 encoded by an oncogenic virus promotes angiogenesis and cellular transformation by enhancing STAT3-mediated epigenetic silencing of caveolin 1
    Article Snippet: Primary human umbilical vein endothelial cells (HUVECs) were cultured in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) as previously described [ 32 ].

    Article Title: HIV-1 Nef and KSHV oncogene K1 synergistically promote angiogenesis by inducing cellular miR-718 to regulate the PTEN/AKT/mTOR signaling pathway
    Article Snippet: Primary human umbilical vein endothelial cells (HUVECs) were cultured and maintained in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) as described ( ).

    Article Title: A KSHV microRNA Directly Targets G Protein-Coupled Receptor Kinase 2 to Promote the Migration and Invasion of Endothelial Cells by Inducing CXCR2 and Activating AKT Signaling
    Article Snippet: HUVEC were cultured in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) and used between passage 3 and 6.

    Article Title: Sperm associated antigen 9 promotes oncogenic KSHV-encoded interferon regulatory factor-induced cellular transformation and angiogenesis by activating the JNK/VEGFA pathway
    Article Snippet: HUVECs were cultured in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) and used between passage 3 and 6.

    Article Title: A KSHV microRNA enhances viral latency and induces angiogenesis by targeting GRK2 to activate the CXCR2/AKT pathway
    Article Snippet: HUVECs were cultured in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) and used between passage 3 and 6.

    Colony Assay:

    Article Title: CircRNA ARFGEF1 functions as a ceRNA to promote oncogenic KSHV-encoded viral interferon regulatory factor induction of cell invasion and angiogenesis by upregulating glutaredoxin 3
    Article Snippet: Primary human umbilical vein endothelial cells (pri-HUVECs), which were used between passages 3 and 6, were isolated from the interior of the umbilical vein of human umbilical cords by digestion with collagenase (Sigma, St. Louis, MO, USA), and cultured in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) as previously described [ ]. pri-HUVECs were used for migration and invasion assays, a human umbilical vein endothelial cell line, EA.hy926 (catalog #CRL-2922; ATCC, Manassas, VA, USA) was employed for RNA-seq analysis, plate colony formation assay and in vivo matrigel plug assay, and HEK293T cells were used for lentivirus packaging and luciferase activity assay.

    Article Title: Viral interleukin-6 encoded by an oncogenic virus promotes angiogenesis and cellular transformation by enhancing STAT3-mediated epigenetic silencing of caveolin 1
    Article Snippet: Primary human umbilical vein endothelial cells (HUVECs) were cultured in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) as previously described [ 32 ].

    Article Title: HIV-1 Nef and KSHV oncogene K1 synergistically promote angiogenesis by inducing cellular miR-718 to regulate the PTEN/AKT/mTOR signaling pathway
    Article Snippet: Primary human umbilical vein endothelial cells (HUVECs) were cultured and maintained in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) as described ( ).

    Article Title: A KSHV microRNA Directly Targets G Protein-Coupled Receptor Kinase 2 to Promote the Migration and Invasion of Endothelial Cells by Inducing CXCR2 and Activating AKT Signaling
    Article Snippet: HUVEC were cultured in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) and used between passage 3 and 6.

    Article Title: Sperm associated antigen 9 promotes oncogenic KSHV-encoded interferon regulatory factor-induced cellular transformation and angiogenesis by activating the JNK/VEGFA pathway
    Article Snippet: HUVECs were cultured in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) and used between passage 3 and 6.

    Article Title: A KSHV microRNA enhances viral latency and induces angiogenesis by targeting GRK2 to activate the CXCR2/AKT pathway
    Article Snippet: HUVECs were cultured in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) and used between passage 3 and 6.

    In Vivo:

    Article Title: CircRNA ARFGEF1 functions as a ceRNA to promote oncogenic KSHV-encoded viral interferon regulatory factor induction of cell invasion and angiogenesis by upregulating glutaredoxin 3
    Article Snippet: Primary human umbilical vein endothelial cells (pri-HUVECs), which were used between passages 3 and 6, were isolated from the interior of the umbilical vein of human umbilical cords by digestion with collagenase (Sigma, St. Louis, MO, USA), and cultured in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) as previously described [ ]. pri-HUVECs were used for migration and invasion assays, a human umbilical vein endothelial cell line, EA.hy926 (catalog #CRL-2922; ATCC, Manassas, VA, USA) was employed for RNA-seq analysis, plate colony formation assay and in vivo matrigel plug assay, and HEK293T cells were used for lentivirus packaging and luciferase activity assay.

    Article Title: Viral interleukin-6 encoded by an oncogenic virus promotes angiogenesis and cellular transformation by enhancing STAT3-mediated epigenetic silencing of caveolin 1
    Article Snippet: Primary human umbilical vein endothelial cells (HUVECs) were cultured in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) as previously described [ 32 ].

    Article Title: HIV-1 Nef and KSHV oncogene K1 synergistically promote angiogenesis by inducing cellular miR-718 to regulate the PTEN/AKT/mTOR signaling pathway
    Article Snippet: Primary human umbilical vein endothelial cells (HUVECs) were cultured and maintained in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) as described ( ).

    Article Title: A KSHV microRNA Directly Targets G Protein-Coupled Receptor Kinase 2 to Promote the Migration and Invasion of Endothelial Cells by Inducing CXCR2 and Activating AKT Signaling
    Article Snippet: HUVEC were cultured in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) and used between passage 3 and 6.

    Article Title: Sperm associated antigen 9 promotes oncogenic KSHV-encoded interferon regulatory factor-induced cellular transformation and angiogenesis by activating the JNK/VEGFA pathway
    Article Snippet: HUVECs were cultured in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) and used between passage 3 and 6.

    Article Title: A KSHV microRNA enhances viral latency and induces angiogenesis by targeting GRK2 to activate the CXCR2/AKT pathway
    Article Snippet: HUVECs were cultured in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) and used between passage 3 and 6.

    Matrigel Assay:

    Article Title: CircRNA ARFGEF1 functions as a ceRNA to promote oncogenic KSHV-encoded viral interferon regulatory factor induction of cell invasion and angiogenesis by upregulating glutaredoxin 3
    Article Snippet: Primary human umbilical vein endothelial cells (pri-HUVECs), which were used between passages 3 and 6, were isolated from the interior of the umbilical vein of human umbilical cords by digestion with collagenase (Sigma, St. Louis, MO, USA), and cultured in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) as previously described [ ]. pri-HUVECs were used for migration and invasion assays, a human umbilical vein endothelial cell line, EA.hy926 (catalog #CRL-2922; ATCC, Manassas, VA, USA) was employed for RNA-seq analysis, plate colony formation assay and in vivo matrigel plug assay, and HEK293T cells were used for lentivirus packaging and luciferase activity assay.

    Article Title: Viral interleukin-6 encoded by an oncogenic virus promotes angiogenesis and cellular transformation by enhancing STAT3-mediated epigenetic silencing of caveolin 1
    Article Snippet: Primary human umbilical vein endothelial cells (HUVECs) were cultured in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) as previously described [ 32 ].

    Article Title: HIV-1 Nef and KSHV oncogene K1 synergistically promote angiogenesis by inducing cellular miR-718 to regulate the PTEN/AKT/mTOR signaling pathway
    Article Snippet: Primary human umbilical vein endothelial cells (HUVECs) were cultured and maintained in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) as described ( ).

    Article Title: A KSHV microRNA Directly Targets G Protein-Coupled Receptor Kinase 2 to Promote the Migration and Invasion of Endothelial Cells by Inducing CXCR2 and Activating AKT Signaling
    Article Snippet: HUVEC were cultured in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) and used between passage 3 and 6.

    Article Title: Sperm associated antigen 9 promotes oncogenic KSHV-encoded interferon regulatory factor-induced cellular transformation and angiogenesis by activating the JNK/VEGFA pathway
    Article Snippet: HUVECs were cultured in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) and used between passage 3 and 6.

    Article Title: A KSHV microRNA enhances viral latency and induces angiogenesis by targeting GRK2 to activate the CXCR2/AKT pathway
    Article Snippet: HUVECs were cultured in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) and used between passage 3 and 6.

    Luciferase:

    Article Title: CircRNA ARFGEF1 functions as a ceRNA to promote oncogenic KSHV-encoded viral interferon regulatory factor induction of cell invasion and angiogenesis by upregulating glutaredoxin 3
    Article Snippet: Primary human umbilical vein endothelial cells (pri-HUVECs), which were used between passages 3 and 6, were isolated from the interior of the umbilical vein of human umbilical cords by digestion with collagenase (Sigma, St. Louis, MO, USA), and cultured in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) as previously described [ ]. pri-HUVECs were used for migration and invasion assays, a human umbilical vein endothelial cell line, EA.hy926 (catalog #CRL-2922; ATCC, Manassas, VA, USA) was employed for RNA-seq analysis, plate colony formation assay and in vivo matrigel plug assay, and HEK293T cells were used for lentivirus packaging and luciferase activity assay.

    Article Title: Viral interleukin-6 encoded by an oncogenic virus promotes angiogenesis and cellular transformation by enhancing STAT3-mediated epigenetic silencing of caveolin 1
    Article Snippet: Primary human umbilical vein endothelial cells (HUVECs) were cultured in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) as previously described [ 32 ].

    Article Title: HIV-1 Nef and KSHV oncogene K1 synergistically promote angiogenesis by inducing cellular miR-718 to regulate the PTEN/AKT/mTOR signaling pathway
    Article Snippet: Primary human umbilical vein endothelial cells (HUVECs) were cultured and maintained in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) as described ( ).

    Article Title: A KSHV microRNA Directly Targets G Protein-Coupled Receptor Kinase 2 to Promote the Migration and Invasion of Endothelial Cells by Inducing CXCR2 and Activating AKT Signaling
    Article Snippet: HUVEC were cultured in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) and used between passage 3 and 6.

    Article Title: Sperm associated antigen 9 promotes oncogenic KSHV-encoded interferon regulatory factor-induced cellular transformation and angiogenesis by activating the JNK/VEGFA pathway
    Article Snippet: HUVECs were cultured in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) and used between passage 3 and 6.

    Article Title: A KSHV microRNA enhances viral latency and induces angiogenesis by targeting GRK2 to activate the CXCR2/AKT pathway
    Article Snippet: HUVECs were cultured in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) and used between passage 3 and 6.

    Activity Assay:

    Article Title: CircRNA ARFGEF1 functions as a ceRNA to promote oncogenic KSHV-encoded viral interferon regulatory factor induction of cell invasion and angiogenesis by upregulating glutaredoxin 3
    Article Snippet: Primary human umbilical vein endothelial cells (pri-HUVECs), which were used between passages 3 and 6, were isolated from the interior of the umbilical vein of human umbilical cords by digestion with collagenase (Sigma, St. Louis, MO, USA), and cultured in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) as previously described [ ]. pri-HUVECs were used for migration and invasion assays, a human umbilical vein endothelial cell line, EA.hy926 (catalog #CRL-2922; ATCC, Manassas, VA, USA) was employed for RNA-seq analysis, plate colony formation assay and in vivo matrigel plug assay, and HEK293T cells were used for lentivirus packaging and luciferase activity assay.

    Article Title: Viral interleukin-6 encoded by an oncogenic virus promotes angiogenesis and cellular transformation by enhancing STAT3-mediated epigenetic silencing of caveolin 1
    Article Snippet: Primary human umbilical vein endothelial cells (HUVECs) were cultured in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) as previously described [ 32 ].

    Article Title: HIV-1 Nef and KSHV oncogene K1 synergistically promote angiogenesis by inducing cellular miR-718 to regulate the PTEN/AKT/mTOR signaling pathway
    Article Snippet: Primary human umbilical vein endothelial cells (HUVECs) were cultured and maintained in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) as described ( ).

    Article Title: A KSHV microRNA Directly Targets G Protein-Coupled Receptor Kinase 2 to Promote the Migration and Invasion of Endothelial Cells by Inducing CXCR2 and Activating AKT Signaling
    Article Snippet: HUVEC were cultured in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) and used between passage 3 and 6.

    Article Title: Sperm associated antigen 9 promotes oncogenic KSHV-encoded interferon regulatory factor-induced cellular transformation and angiogenesis by activating the JNK/VEGFA pathway
    Article Snippet: HUVECs were cultured in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) and used between passage 3 and 6.

    Article Title: A KSHV microRNA enhances viral latency and induces angiogenesis by targeting GRK2 to activate the CXCR2/AKT pathway
    Article Snippet: HUVECs were cultured in complete EBM-2 culture media (LONZA, Allendale, NJ, USA) and used between passage 3 and 6.



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